Device development and microfluidics
Device development involves development of reliable technologies for fabrication and scale-up. One of the important aspects our work involves development of a robust machine and allied processes to fabricate U-bent fiberoptic probes. Automated fiber bending system for mass production of the U-bent probes is one such technology that was developed in-house at IIT Madras. A fiber probe fabrication facility is set-up at IIT Madras to cater to the needs of the researchers in academia as well as industry. We have a capacity to bend 200 and 400 micron core silica fibers as many as 50 probes in an hour with high reproducibility. We have also established a process for fabrication of U-bent probes using plastic optical fiber (POF). A patent has been filed to protect the intellectual property and allow technology transfer for commercialization. This development would hopefully allow much more wider applications of the versatile U-bent probes including plasmonic chemical and bio sensing, absorbance measurements and refractive index measurements. Fiber optic refractometer (FOR) is another technology for development of a hand-held device to utilize the U-bent probes as sensors for measurement of refractive index of a given liquid. In addition, this device would also be helpful in using them for other chemical and bio sensing applications.
Decladding: The fiber probe region was
cleaned by dipping it in acetone (5 min) followed by MeOH + HCl
treatment for 5 min to remove traces of any polymer cladding
or other organic contamination on the surface.
Surface functionalization & Gold nanoparticle immobilization: These fiber probes were immersed in sulphochromic solution (100 ml conc. H2SO4 + 1 ml of 0.5 mg/ml K2Cr2O7) for 10 min, washed in DI water and dehydrated for 2 h at 115 ◦C to generate silanol sites). Fibers were silanized by dipping in 1% aminosilane in ethanol: acetic acid (10:4 v/v) mixture for 5 min and washed thrice in ethanol to generate amine functional groups. The pH of silane solution was maintained at 3.4 in order to minimize adsorption of protonated amine groups with silanol sites. These fiber probes were placed in a hot-air oven at 110 ◦C for 20 min for condensation. Gold nanoparticles could be immobilized on amine functionalized probes.
Go to the publication
Bending: POF comprises of polymethylmethacrylate (PMMA) and fluorinated
polymer as core and cladding having refractive indices of 1.49and 1.41 respectively.
The fiber wasbent in order to bring both ends closer and pushed inside a 15 cm
long glass capillary tube of varying inner diameter.
The capillary tube was placed in a hot air oven for 10 min at 100◦C.
Here, fibers are bent by placing them in the hot ambience without subjecting them to a
blow of hot air directly, to reduce thermal stresses.
Decladding: The U-bent probes were prepared by precisely decladding the fluorinated polymer layer over the U-bent region by chemical etching to sensitize the core surface for evanescent wave based sensing.These probes were decladded by dipping in a vertical flow cell of smaller cross sectional area which was completely filled with ethylacetate for 2 min.
Surface functionalization & Gold nanoparticle immobilization: The surface of PMMA fiber probe was amine functionalized using Hexamethylenediamine as described by Fixe et al. Gold nanoparticles are immobilized onto the amine functionalized probe to generate an plasmonically active U-bent RI sensor.
The sensitivity of the U-bend probes, depends greatly on the bend diameter of the probe. Experimental and theoretical investigations have been carried out to understand light propagation in U-bent optical fiber and to estimate the bend diameter for achieving maximum sensitivity.
These probes are effective low-cost refractive index sensors. They could also be used for force, pressure and level sensing. There is a pressing need for a simple and portable milk quality detection, since food adulteration is one of the major problems in India. These probes are being tested for determination of fat percentage and presence of adulterants such as urea or sucrose.